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As all the progenies developed by hybridization, it is supposed that they shared common genetic basis.

Quality of DNA was assessed by electrophoresis on 0.8 % agarose gel and its quality and quantity was evaluated in Nanodrop ND-1000 spectrophotometer (Nanodrop Technologies, Wilmington, Dalware) to dilute DNA stock as 15 ng/µL d (25 m M), Primer (Forward and Reverse) 10× from 100× (1.0 µL for each), Taq DNA polymerase 0.2 and 5.8 µL of nuclease-free water.

Increasingly, 38.46 % of these loci were scored as homozygous alleles while 61.53 % heterozygous allelic loci were determined.

Primer mpd CIR48 produced a specific locus (250/250) in all male samples whereas the same locus was absent in female samples.

However, the lack of molecular markers in date palm restricts the application of molecular breeding.